p cjun  (Cell Signaling Technology Inc)

Journal: Frontiers in Cellular Neuroscience

Article Title: Microglial mechanisms of viable retinal ganglion cell elimination

doi: 10.3389/fncel.2025.1719791

Figure Lengend Snippet: A subpopulation of RGCs express the stress marker phosphorylated c-JUN (p-cJUN). (A) Whole mount image ( Cx3cr1-Gfp/+ ) of e14.5 retina showing that a subset of RGCs (Brn3a+; red) express nuclear p-cJUN (white; yellow arrows). Boxed regions show enlarged region and label for p-cJUN alone or Brn3a alone (A′) . Scale bar = 10 μm. (B) Whole mount image (e14.5 Cx3cr1-Gfp/+ ) showing microglia (GFP) internalizing an RGC (Brn3a + red) labeled with p-cJUN (white) using HCR IHC. Scale bar = 1 μm. (C) Whole mount images showing RGCs (Brn3a+; red) with nuclear p-cJUN (white) in control mice (WT) and Mertk/CR3 dKO mice. Yellow arrows indicate double positive p-cJUN and Brn3a staining. (D) Quantification of double positive p-cJUN + Brn3a + RGCs per mm 2 in e14.5 control and Mertk/CR3 dKO mice. *** p < 0.01 unpaired t -test [ N = 4 (WT), 5 (Mertk CR3 dKO)]. (E) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in e14.5 and e16.5 Mertk/CR3 dKO mice. * p < 0.05 Welch’s t -test [ N = 5 (e14.5), 4 (e16.5)]. (F) Quantification of p-cJUN + Brn3a + RGCs per mm 2 in P0 control, Mertk KO and Mertk/CR3 dKO mice. ns, not significant. Welch’s one-way ANOVA with Brown–Forsythe variance test [ N = 7 (WT), 7 (Mertk KO), 6 (Mertk CR3 dKO)]. (G) Whole mount retina images to show vehicle treated Axl KO mice with RGCs (Brn3a+; red) expressing nuclear p-cJUN (white) and microglia present (IBA1+; green). Scale bar = 500 μm. Boxed regions show enlarged area (G′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100-10 μm. (H) Whole mount retina images to show Axl KO PLX treated mice with depletion of microglia (IBA1; green) and RGCs (Brn3a+; red) with nuclear p-cJUN (white) expression remaining. Boxed regions show enlarged area (H′) with yellow arrows indicating double positive p-cJUN and Brn3a staining. Scale bars = 100 to 10 μm.

Article Snippet: Antibody , Rabbit anti p-cJUN , Cell Signaling , 1:500 , 9261S.

Techniques: Marker, Labeling, Control, Staining, Expressing

Journal: Cell Death Discovery

Article Title: Atorvastatin improves spermatogenesis in murine and in vitro human chronic orchitis models through restoring blood-testis barriers

doi: 10.1038/s41420-025-02749-6

Figure Lengend Snippet: a Venn diagram of differential expressed proteins (DEPs) between groups. b Enriched GO terms of the co-downregulated proteins in Vehicle and T + AT groups compared with TNF-α group. c Heatmap showing the top 10 co-downregulated proteins according to the fold change between T + AT group and TNF-α group. d Western blot images of MMP3 and MMP9 in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups. e Grayscale value analysis of western blot for MMP3 and MMP9 in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups, n = 3. f , g Immunofluorescence of MMP3 and MMP9 (green) co-stained with SOX9 (red) in Vehicle, EAO-Sal and EAO-AT groups and quantitative analysis for immunofluorescence intensity of MMP3 and MMP9 in SOX9 + Sertoli cells from 20 randomly selected fields. SOX9 labeled Sertoli cells. Scale bar, 10 μm. n = 4 testis samples per group. h Western blot assay of Rac1, cJUN and Phospho-cJUN (p-cJUN) in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups. i Grayscale value analysis of western blot for Rac1 and p-cJUN in TM4 Sertoli cells from Vehicle, TNF-α and T + AT groups, n = 3. j Immunofluorescence of cJUN (green) co-stained with SOX9 (red) in Vehicle, EAO-Sal and EAO-AT groups and quantitative analysis for immunofluorescence intensity of cJUN in the nucleus of SOX9 + Sertoli cells from 20 randomly selected fields. SOX9 labeled Sertoli cells. Scale bar, 10 μm. k Western blot images of MMP3, MMP9, CX43 and ZO1 in TM4 Sertoli cells after T-5224 treatment. Data are presented as mean ± SEM, * P < 0.05, ** P < 0.01, **** P < 0.0001, ns indicates no statistical significance.

Article Snippet: The primary antibodies used in this study were listed: rabbit anti-HMGCR (A19063, ABclonal, 1:1000), rabbit anti-CX43 (26980-1-AP, Proteintech, 1:1000), rabbit anti-ZO1 (21773-1-AP, Proteintech, 1:1000), rabbit anti-CTNNB1 (ab32572, Abcam, 1:1000), rabbit anti-MMP3 (17873-1-AP, Proteintech, 1:1200), rabbit anti-MMP9 (10375-2-AP, Proteintech, 1:1200), rabbit anti-cJUN (ab32137, Abcam, 1:4000), rabbit anti-p cJUN (9261S, Cell Signaling Technology, 1:1000), rabbit anti-Rac1 (24072-1-AP, Proteintech, 1:1000).

Techniques: Western Blot, Immunofluorescence, Staining, Labeling